u2os mg63 cell line  (CHI Scientific Inc)

Journal: Frontiers in Pharmacology

Article Title: A Systematic Review of the Preventive and Therapeutic Effects of Naringin Against Human Malignancies

doi: 10.3389/fphar.2021.639840

Figure Lengend Snippet: Potential anticancer effects and related mechanisms of action of naringin based on in vitro studies.

Article Snippet: Sarcoma (osteosarcoma) , MG63 and U2OS cell lines , 10–20 μmol/L , Beyotime Biotechnology (Shanghai, China) , ND , Y , 24 h , ↓Cell proliferation, ↓cell invasion, ↑apoptosis, ↓Zeb1, ↓cell migration, ↑cell cycle arrest , .

Techniques: In Vitro, Control, Activity Assay, Inhibition, Protein-Protein interactions, Transduction, Purification, Mass Spectrometry, Migration, High Performance Liquid Chromatography

Journal: Frontiers in Pharmacology

Article Title: A Systematic Review of the Preventive and Therapeutic Effects of Naringin Against Human Malignancies

doi: 10.3389/fphar.2021.639840

Figure Lengend Snippet: Potential anticancer effects and related mechanisms of action of naringin based on in vivo studies.

Article Snippet: Sarcoma (osteosarcoma) , MG63 and U2OS cell lines , 10–20 μmol/L , Beyotime Biotechnology (Shanghai, China) , ND , Y , 24 h , ↓Cell proliferation, ↓cell invasion, ↑apoptosis, ↓Zeb1, ↓cell migration, ↑cell cycle arrest , .

Techniques: In Vivo, Animal Model, Control, Purification, Liquid Chromatography

Journal: Journal of Orthopaedic Surgery and Research

Article Title: URG4 mediates cell proliferation and cell cycle in osteosarcoma via GSK3β/β-catenin/cyclin D1 signaling pathway

doi: 10.1186/s13018-020-01681-y

Figure Lengend Snippet: Increased expression of URG4 in osteosarcoma tissues and cell lines. a URG4 expression was significantly increased in osteosarcoma tissues than corresponding normal tissues by HE and IHC, respectively (× 200 magnification). b PCR determined URG4 mRNA expression in osteosarcoma cell lines (HOS, MG63, Saos-2, U2OS, and 143B), and hFOB 1.19 was used as control. c Western blot assay determined URG4 protein expression in osteosarcoma cell lines (HOS, MG63, Saos-2, U2OS, and 143B), and hFOB 1.19 was used as control. d The mRNA expression level of the URG4 in HOS and MG63 cell lines following transfection as determined by RT-qPCR. e The protein expression level of URG4 in HOS and MG63 cell lines following transfection as determined by western blot assay. HE: hematoxylin and eosin; IHC: Immunohistochemistry; URG4: upregulated gene 4; Normal: normal tissues; OS: osteosarcoma tissues; K: blank group; NC: negative control. * p < 0.05, ** p < 0.01 vs the NC

Article Snippet: Five osteosarcoma cell lines (MNNG/HOS, 143B, U2OS, Saos-2, and MG63) and a human osteoblast cell line (hFOB 1.19) were purchased from Shanghai Genechem Company Ltd. U2OS, 143B, and MG63 cells were cultured in DMEM (Gibco, USA), whereas MNNG/HOS and Saos-2 cells were cultured in RPMI 1640 medium. hFOB 1.19 cells were cultured in 1:1 DMEM/Ham’s F12 medium supplemented with 2.5 mM l -glutamine and 0.3 mg/ml G418 (Sigma-Aldrich, St Louis, MO, USA).

Techniques: Expressing, Control, Western Blot, Transfection, Quantitative RT-PCR, Immunohistochemistry, Negative Control

Journal: Journal of Orthopaedic Surgery and Research

Article Title: URG4 mediates cell proliferation and cell cycle in osteosarcoma via GSK3β/β-catenin/cyclin D1 signaling pathway

doi: 10.1186/s13018-020-01681-y

Figure Lengend Snippet: URG4 affects vitro migration, invasion, colony formation, and vivo tumorigenesis. a HOS and MG63 cells stably transfected with siURG4 or siCtrl, and migration ability was evaluated by wound-healing assay. b Transwell assay was performed to analyze invasive ability. c Colony-forming abilities were researched by plate clones assay. d Images, volume, and weight of transplanted tumors in nude mice 31 days after subcutaneous inoculation with osteosarcoma cells stably expressing siURG4-1 or si-NC. URG4: upregulated gene 4; K: blank group; NC: negative control. * p < 0.05, ** p < 0.01 vs the NC

Article Snippet: Five osteosarcoma cell lines (MNNG/HOS, 143B, U2OS, Saos-2, and MG63) and a human osteoblast cell line (hFOB 1.19) were purchased from Shanghai Genechem Company Ltd. U2OS, 143B, and MG63 cells were cultured in DMEM (Gibco, USA), whereas MNNG/HOS and Saos-2 cells were cultured in RPMI 1640 medium. hFOB 1.19 cells were cultured in 1:1 DMEM/Ham’s F12 medium supplemented with 2.5 mM l -glutamine and 0.3 mg/ml G418 (Sigma-Aldrich, St Louis, MO, USA).

Techniques: Migration, Stable Transfection, Transfection, Wound Healing Assay, Transwell Assay, Clone Assay, Expressing, Negative Control

Journal: Journal of Orthopaedic Surgery and Research

Article Title: URG4 mediates cell proliferation and cell cycle in osteosarcoma via GSK3β/β-catenin/cyclin D1 signaling pathway

doi: 10.1186/s13018-020-01681-y

Figure Lengend Snippet: URG4 regulates osteosarcoma cell growth by activating the GSK3β/β-catenin/cyclin D1 signaling pathway. a URG4-related genes enriched in the top 10 significant signaling pathway based on KEGG. b The protein level of GSK3β, p-GSK3β, nuclear β-catenin, and cyclin D1 in the HOS cell transfected with siURG4 was revealed by the western blot assay. c The protein level of GSK3β, p-GSK3β, nuclear β-catenin, and cyclin D1 in the MG63 cell transfected with siURG4 was revealed by the western blot assay. d The positive expression of the nuclear β-catenin protein in osteosarcoma cells was measured by the immunohistochemistry assay. e A model depicted URG4 promotes osteosarcoma progression via the GSK3β/β-catenin/cyclin D1 pathway. URG4: upregulated gene 4; NC: negative control. * p < 0.05 vs the NC

Article Snippet: Five osteosarcoma cell lines (MNNG/HOS, 143B, U2OS, Saos-2, and MG63) and a human osteoblast cell line (hFOB 1.19) were purchased from Shanghai Genechem Company Ltd. U2OS, 143B, and MG63 cells were cultured in DMEM (Gibco, USA), whereas MNNG/HOS and Saos-2 cells were cultured in RPMI 1640 medium. hFOB 1.19 cells were cultured in 1:1 DMEM/Ham’s F12 medium supplemented with 2.5 mM l -glutamine and 0.3 mg/ml G418 (Sigma-Aldrich, St Louis, MO, USA).

Techniques: Transfection, Western Blot, Expressing, Immunohistochemistry, Negative Control